王凤雪(1978-),山东潍坊人,博士,副教授,硕士研究生导师,中共党员,美国农业部国家动物疾病中心访问学者,内蒙古农业大学高层次人才(第五层次A类),内蒙古自治区“高校青年科技英才”。主讲《兽医微生物学》、《畜牧微生物学》和《兽医免疫学》。目前共发表中英文论文120余篇,在业内主流刊物Veterinary Sciences,Frontiers in Microbiology,Scientific Reports,Veterinary Microbiology,Infection, genetics and evolution等杂志上发表英文SCI论文29篇,SCI论文第一或通讯作者17篇,合计影响因子49.741。参编专业著作2部,发明专利2项,获科技奖励共10项。曾主持和参加完成国家科技支撑计划项目、国家863项目、农业部兽医局国际合作项目和省、市科研等各类项目共20余项;主持在研项目4项。
教育工作经历:
1998.9-2002.7 莱阳农学院兽医专业毕业 农学学士
2002.9-2005.12中国农业科学院哈尔滨兽医研究所预防兽医学专业毕业农学硕士
2008.9-2012.1中国农业科学院特产研究所预防兽医学专业毕业农学博士
2005.7-2008.12中国农业科学院特产研究所 研习员
2009.1-2012.12中国农业科学院特产研究所 助理研究员
2013.1-2018.02中国农业科学院特产研究所 副研究员
2016.1-2017.12美国农业部国家动物疾病中心 访问学者
2018.3-至今 内蒙古农业大学兽医学院 副研究员
研究方向:
1.基础研究方面,猪蓝耳病病毒致病和免疫抑制机制研究。
2.应用研究方面,病原微生物疫苗及载体研究,利用反向遗传学技术和基因编辑技术研制基因工程疫苗,成功建立猪蓝耳病病毒、牛副流感病毒、犬瘟热病毒、狂犬病毒、一型疱疹病毒和布鲁菌等的基因操作平台。
3.应用技术方面,以主要负责人研发获得猪蓝耳病病毒缺陷型疫苗毒候选株(筛选验证阶段),参与建立家畜多种疫病病原的诊断试剂研发。
主要研究项目(负责人):
1.内蒙古直属高校基本科研业务费项目“内蒙地区重要猪病毒病生物制品研发级流行病学调查”(BR220113),15万元,2022.08-2025.07
2.内蒙古自治区教育厅“高等学校青年科技英才”项目(NJYT22043),30万元,2022.01-2024.12
3.内蒙古自治区自然基金项目“表达PRRSV ORF2/ORF4基因的Marc-145细胞系的建立及复制缺陷型疫苗候选毒的研制”(2020MS03047)6.5万元,2020.01-2022.12。
4.内蒙古农业大学高层次人才科研启动项目(NDYB2018),100万元,2019.01-2022.12
学术任职:
2013年1月-至今 《病毒学报》 审稿专家
2017年11月-至今Transboundary and Emerging Diseases审稿专家
2021年11月-至今Microbial Pathogenesis审稿专家
2022年4月-至今MDPIVirus和Animals审稿专家
主要论文成果:
1.Hou LN1,Wang FX1,Wang YX, Guo H, Liu CY, Zhao HZ, Yu MH, Wen YJ. Subunit vaccine based on glycoprotein B protects pattern animal guinea pigs from tissue damage caused by infectious bovine rhinotracheitis virus. Virus Res. 2022, 320:198899. doi: 10.1016/j.virusres.2022.198899.(中科院JCR分区3区,IF:6.286)
2.Liu, C.-Y.; Guo, H.; Zhao, H.-Z.; Hou, L.-N.;Wen, Y.-J.*;Wang, F.-X*(通讯作者).Recombinant Bovine Herpesvirus Type I Expressing the Bovine Viral Diarrhea Virus E2 Protein Could Effectively Prevent Infection by Two Viruses. Viruses 2022, 14, 18. https://doi.org/10.3390/v14081618.(中科院JCR分区2区,IF:5.818)
3.Zhao H-z1,Wang F-x1,Han X-y, Guo H, Liu C-y, Hou L-n, Wang Y-x, Zheng H, Wang L and Wen Y-j*. Recent advances in the study of NADC34-like porcine reproductive and respiratory syndrome virus in China. Front. Microbiol. 2022,13: 950402. doi: 10.3389/fmicb.2022.950402.(中科院JCR分区2区,IF:6.064.TOP期刊)
4.Liu, C.-Y.; Jin, M.; Guo, H.; Zhao, H.-Z.; Hou, L.-N.; Yang, Y.; Wen, Y.-J*.;Wang, F.-X*(通讯作者). Concurrent Gene Insertion, Deletion, and Inversion during the Construction of a Novel Attenuated BoHV-1 Using CRISPR/Cas9 Genome Editing. Vet. Sci. 2022, 9, 166. https://doi.org/ 10.3390/vetsci9040166.(中科院JCR分区3区,IF:2.518)
5.Wang FX,Zhang HZ, Hou LN, Yang C, Wen YJ: Advance of African swine fever virus in recent years. Research in veterinary science 2021, 136:535-539.(中科院JCR分区3区,IF:2.554)
6.Yin, K., Li, Y., Ma, Z., Yang, Y., Zhao, H., Liu, C., Jin, M., Wudong, G., Sun, Y., Hang, T., Zhang, H.,Wang, F.(通讯作者)*, Wen, Y.,* SNAP25 regulates the release of the Rabies virus in nerve cells via SNARE complex-mediated membrane fusion. Veterinary microbiology,2020, 245: 108699. (中科院JCR分区1区,IF:3.03)
7.Feng-Xue Wang,Yong Yang, Xing Liu, Min-Hui He, Ying Liu, Na Sun, Hong-Wei Zhu, Jing-Qiang Ren, Hua Wu, Yong-Jun Wen.Development of monoclonal antibody for differentiating porcine reproductive and respiratory syndrome virus and identification of a novel non-structural protein 2 epitope peptide. Virusdisease, 2017 Dec;28(4):408-415. doi: 10.1007/s13337-017-0400-x. (中科院JCR分区4区)
8.Wang, F.X.,Yuan D.Y.,Jin Y.N., HuL., Sun Z.Y., He Q.,Zhao S.H., Zhan S.B., Wen Y.J.Reverse Transcription Cross-Priming Amplification-Nucleic Acid Test Strip for Rapid Detection of Porcine Epidemic Diarrhea Virus[J]. Scientific Reports, 6(24702) , 2016. (中科院JCR分区3区, IF: 4.259)
9.Wang, F.X.,Qin, L.T., Liu, Y., Liu, X., Sun, N., Yang, Y., Chen, T., Zhu, H.W., Ren, J.Q., Sun, Y.J., Cheng, S.P., Wen, Y.J. Novel Nsp2 deletion based on molecular epidemiology and evolution of porcine reproductive and respiratory syndrome virus in Shandong Province from 2013 to 2014[J]. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2015, 33(219-226). (中科院JCR分区3区,IF: 2.591)
10.Yang, Y.1,Wang, F.X.1,Sun, N., Cao, L., Zhang, S.Q., Zhu, H.W., Guo, L., Cheng, S.P., Wen, Y.J. Development and evaluation of two truncated recombinant NP antigen-based indirect ELISAs for detection of bovine parainfluenza virus type 3 antibodies in cattle[J]. J virol methods, 2015, 222(47-54. (中科院JCR分区4区,IF: 1.508)
11.Wang, F.X.,Zhang, S.Q., Zhu, H.W., Yang, Y., Sun, N., Tan, B., Li, Z.G., Cheng, S.P., Fu, Z.F., Wen, Y.J. Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge[J]. Vet Microbiol, 2014, 174(3-4): 362-371. (中科院JCR分区1区, IF: 2.511)
12.Wang, F.-X.,Guo, L., Yang, Y.-L., Song, N., Chen, L.-Z., Cheng, S.-P., Wen, Y.-J. Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China[J]. Indian J. Virol., 2013, 24(1): 90-92. (IF : 0.364)
13.Wang, F.X.,Song, N., Chen, L.Z., Cheng, S.P., Wu, H., Wen, Y.J. Non-structural protein 2 of the porcine reproductive and respiratory syndrome (PRRS) virus: A crucial protein in viral pathogenesis, immunity and diagnosis[J]. Res Vet Sci, 2013, 95(1): 1-7. (中科院JCR分区3区, IF: 1.511)
14.WANG, F.-X., WEN, Y.-J., YANG, B.-C., LIU, Z., SHI, X.C., LENG, X., SONG, N., WU, H., CHEN, L.-Z., CHENG, S.-P. Role of non-structural protein 2 in the regulation of the replication of the porcine reproductive and respiratory syndrome virus in MARC-145 cells: effect of gene silencing and over expression[J]. Vet. Microbiol., 2012, 161:58-65. (中科院JCR分区1区, IF: 3.127)
15.Wang, F.,Yan, X., Chai, X., Zhang, H., Zhao, J., Wen, Y., Wu, W. Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china[J]. Virology journal, 2011, 8:85. (中科院JCR分区3区,IF: 2.343)
16.Yang, B.C.1,Wang, F.X.1,Zhang, S.Q., Song, N., Li, J.X., Yang, Z.Q., Wen, Y.J., Wu, H. Comparative evaluation of conventional polymerase chain reaction (PCR), with loop-mediated isothermal amplification and SYBR green I-based real-time PCR for the quantitation of porcine circovirus-1 DNA in contaminated samples destined for vaccine production[J]. J. Virol. Methods, 2013, 191(1): 1-8. (中科院JCR分区4区, IF: 1.883)
17.Spear, A.,Wang, F-X,Kappes, M.A, Das, P.B., and K. S. Faaberg. 2018. Progress to an Enhanced Vaccine: Eight Marked Attenuated Viruses to Porcine Reproductive and Respiratory Disease Virus. Virology 515, 30-37. https://doi.org/10.1016/j.virol.2017.1 (中科院JCR分区3区, IF:3.374)
18.Liu,X.,Wang, F.X.,Zhu, H.W., Sun, N. Wu, H. Phylogenetic analysis of porcine circovirus type 2(PCV2) isolates from China with high homology to PCV2c[J]. Archives of Virology, 2016, 161:1591-1599. (中科院JCR分区4区,IF: 2.39)
19.Liu Y.,Wang, F.X.,Wen, Y.J., Li, Z.G., Liu, X., Sun, N., Yang, Y., Zhang, S.Q., Zhu, H.W., Cheng, S.P., Wu, H. Effect of non-structural protein 2 hypervariable regions in the replication of porcine reproductive and respiratory syndrome virus in MARC-145 cells[J]. Intervirology, 2015,58(5): 288-296. (中科院JCR分区4区,IF: 1.011)
20.Wang FX, Liu Y, Zhu HW, Liu X, Yang Y, Sun N, Cheng SP, Wen YJ. Safety of inoculation of bovine parainfluenza virus 3 as potential vaccine vector in pigs. Virusdisease. 2015 Jun;26(1-2):89-91. doi: 10.1007/s13337-015-0250-3. (中科院JCR分区4区)
21.Wang, F.-X., Yang, B.-C., Wen, Y.-J., Liu, Z., Leng, X., Shi, X.-C., Wang, W., Li, Z.-G., Tan, B., Chen, L.-z., Cheng, C.-p., Wu, H. Genomic characterization of porcine reproductive and respiratory syndrome virus TJM vaccine strain[J]. International Research Journal of Microbiology, 2012, 3(5): 191-201.
22.王亚新,刘春羽,侯丽娜,温永俊*,王凤雪*..牛传染性鼻气管炎病毒SYBR GreenⅠ荧光定量PCR检测方法的建立[J].动物医学进展,2022,43(11):6-11.DOI:10.16437/j.cnki.1007-5038.2022.11.013.
23.张赫,杭天宇,刘春羽,尹坤,温永俊*,王凤雪*.非洲猪瘟病毒CP204L基因的原核表达与单抗制备[J].病毒学报,2021,37(02):370-375.
24.郭昊,乌东高娃,赵洪哲,刘春羽,侯丽娜,王凤雪*,温永俊*.PRRSV GP4蛋白的原核表达及单克隆抗体的制备[J/OL].中国动物传染病学报,2022.
25.杨俊杰,韩晓语,刘春羽,王亚新,郭昊,温永俊*,王凤雪*.鸽I型腺病毒SYBR Green I荧光定量PCR检测方法的建立[J].中国预防兽医学报,2022,44(10):1066-1070.
26.侯丽娜,于明华,刘春羽,赵洪哲,郑秉武,温永俊*,王凤雪*.牛传染性鼻气管炎病毒gI蛋白截短表达与单克隆抗体制备[J].中国动物检疫,2022,39(08):112-119.
27.王凤雪,冷雪,李真光,温永俊,刘准,武华.高致病性PRRSV TJ株Nsp2蛋白的缺失及其二级结构预测[J].湖南农业大学学报(自然科学版), 2011, 37(04): 429-435.
28.王凤雪,郭利,温永俊,宋妮,杨艳玲,张淑琴,谭斌,武华.吉林地区高致病性PRRSV JL-04/12株的分离鉴定与全基因组测序[J].西北农林科技大学学报(自然科学版), 2013, 04): 37-44.
29.王凤雪,刘准,温永俊,冷雪,李真光,武华.表达PRRSV强弱毒Nsp2的Marc-145细胞系的建立及Nsp2蛋白对PRRSV复制的影响[J].西北农业学报, 2012, 21(03): 1-6.
30.王凤雪,师新川,温永俊,胡嘉欣,刘准,武华.高致病性PRRSVNsp2基因RNA干扰对病毒复制的影响[J].生物技术通报, 2012, 05): 132-137.
31.王凤雪,温永俊,武华.猪繁殖与呼吸综合征病毒感染相关受体的研究概况[J].黑龙江畜牧兽医, 2010, 9(17): 44-46.
32.王凤雪,温永俊,刘准,冷雪,李真光,武华.高致病性PRRSV TJ株和致弱毒TJM株Nsp2测序及结构分析[J].河南农业大学学报, 2011, 45(01): 86-90.